CYP2R1

CYP2R1
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 3C6G, 3CZH, 3DL9
Identifiers
Aliases	CYP2R1, cytochrome P450 family 2 subfamily R member 1
External IDs	OMIM: 608713 MGI: 2449771 HomoloGene: 75210 GeneCards: CYP2R1
Gene location (Human) Chr. Chromosome 11 (human)[1] Band 11p15.2 Start 14,877,440 bp[1] End 14,892,231 bp[1]
Gene location (Mouse) Chr. Chromosome 7 (mouse)[2] Band 7|7 F1 Start 114,148,917 bp[2] End 114,162,207 bp[2]
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in sperm body of pancreas skin of abdomen body of stomach rectum ascending aorta minor salivary glands jejunal mucosa tibia Achilles tendon Top expressed in left lobe of liver spermatocyte secondary oocyte seminiferous tubule proximal tubule spermatid duodenum esophagus lip stomach More reference expression data BioGPS More reference expression data
Gene ontology Molecular function iron ion binding metal ion binding monooxygenase activity heme binding oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen oxidoreductase activity vitamin D3 25-hydroxylase activity steroid hydroxylase activity oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen Cellular component organelle membrane endoplasmic reticulum membrane intracellular membrane-bounded organelle membrane endoplasmic reticulum cytoplasm Biological process response to ionizing radiation vitamin D metabolic process response to metal ion vitamin metabolic process calcitriol biosynthetic process from calciol response to cesium ion organic acid metabolic process xenobiotic metabolic process vitamin D biosynthetic process Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 120227 244209 Ensembl ENSG00000186104 ENSMUSG00000030670 UniProt Q6VVX0 Q6VVW9 RefSeq (mRNA) NM_024514 NM_001377214 NM_001377215 NM_001377216 NM_001377217 NM_001377227 NM_177382 RefSeq (protein) NP_078790 NP_001364143 NP_001364144 NP_001364145 NP_001364146 NP_001364156 NP_796356 Location (UCSC) Chr 11: 14.88 – 14.89 Mb Chr 7: 114.15 – 114.16 Mb PubMed search [3] [4]
Wikidata
View/Edit Human View/Edit Mouse

CYP2R1 is cytochrome P450 2R1, an enzyme which is the principal vitamin D 25-hydroxylase.^[5][6] In humans it is encoded by the CYP2R1 gene located on chromosome 11p15.2.^[7] It is expressed in the endoplasmic reticulum in liver, where it performs the first step in the activation of vitamin D by catalyzing the formation of 25-hydroxyvitamin D.^[8]

Vitamin D 25-hydroxylase activity is also possessed by some other cytochrome P450 enzymes, in particular CYP27A1, which is found in mitochondria.^[8][9]

Function

CYP2R1 is a member of the cytochrome P450 superfamily of enzymes.^[10] The cytochrome P450 proteins are mono-oxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids.^[10]

CYP2R1 is present in the endoplasmic reticulum of the liver (the microsomal fraction). It has 25-hydroxylase activity, which converts cholecalciferol (vitamin D₃) into calcifediol (25-hydroxyvitamin D₃, also known as calcidiol), the major circulatory form of the vitamin.^[8][9] CYP2R1 will also hydroxylate ergocalciferol (vitamin D₂), derived from dietary sources, into 25-hydroxyvitamin D₂ (ercalcidiol).^[8] These 25-hydroxylated forms of vitamin D, together known as 25(OH)D, bind strongly to the vitamin D-binding protein in blood and are the principal circulating forms of vitamin D. These are commonly measured to determine a person's vitamin D status and establish vitamin D deficiency.^[11]

Calcifediol is subsequently converted by the action of 25-hydroxyvitamin D3 1-alpha-hydroxylase to calcitriol, the active form of vitamin D₃ which binds to the vitamin D receptor (VDR) and mediates most of the physiological hormonal actions of vitamin D.^[5]

Clinical significance

The conversion of vitamin D, especially cholecalciferol, to 25(OH)D (calcifediol) is one of the key steps in the vitamin D hormonal system. The CYP2R1 enzymatic activity achieving this process was previously thought to be constitutively expressed and stable, so that serum 25(OH)D was a measure of the supply of vitamin D.^[9]

CYP2R1 is now known to be regulated, with variations in the expression and activity of CYP2R1 affecting circulating 25(OH)D.^[9] Low levels of CYP2R1 activity have been found after 24 hour fasting, in obesity, type 1 and type 2 diabetes^[12] and are decreased by glucocorticoids such as dexamethasone.^[9] These conditions are known to be linked to low blood levels of 25(OH)D, where even large doses of vitamin D may not produce an improvement, which can be explained by enzyme activities being low.^[9]

Polymorphic variations in CYP2R1

Polymorphic variations in the CYP2R1 gene have the greatest effect on individual serum 25(OH)D concentrations compared with other gene variations.^[13] An inherited mutation in the CYP2R1 gene L99P, which results in the substitution of a proline for a leucine residue at codon 99, eliminates the enzyme activity and is associated with vitamin D-dependent rickets type IB. Another variant is K242N, where lysine at position 242 is substituted by asparagine, give a similar phenotype.^[14] Symptoms are low circulating levels of 25(OH)D and classic symptoms of vitamin D deficiency.^[5][15]

Interactive pathway map

Click on genes, proteins and metabolites below to link to respective articles. ^{[§ 1]}

[[File:

[[

]]

[[

]]

[[

]]

[[

]]

[[

]]

[[

]]

[[

]]

[[

]]

[[

]]

[[

]]

[[

]]

[[

]]

[[

]]

[[

]]

[[

]]

[[

]]

[[

]]

[[

]]

[[

]]

[[

]]

[[

]]

[[

]]

|alt=Vitamin D Synthesis Pathway (view / edit)]]

Vitamin D Synthesis Pathway (view / edit)

1. ^ The interactive pathway map can be edited at WikiPathways: "VitaminDSynthesis_WP1531".

Studies in mice

Model organisms have been used in the study of CYP2R1 function. Mice have been generated with knockout of Cyp2r1 and both Cyp2r1 and Cyp27a1.^[16] A conditional knockout mouse line called Cyp2r1^{tm1b(EUCOMM)Wtsi} has been generated and animals have undergone a standardized phenotypic screen.^[17][18]

References

External links

• Human CYP2R1 genome location and CYP2R1 gene details page in the UCSC Genome Browser.