Cathepsin L1

Protein-coding gene in the species Homo sapiens
CTSL
Available structures
PDBHuman UniProt search: PDBe RCSB
List of PDB id codes

1CJL, 1CS8, 1ICF, 1MHW, 2NQD, 2VHS, 2XU1, 2XU3, 2XU4, 2XU5, 2YJ2, 2YJ8, 2YJ9, 2YJB, 2YJC, 3BC3, 3H89, 3H8B, 3H8C, 3HHA, 3HWN, 3IV2, 3K24, 3KSE, 3OF8, 3OF9, 4AXL, 4AXM, 5F02, 5I4H

Identifiers
AliasesCTSL, CATL, CTSL1, MEP, cathepsin L
External IDsOMIM: 116880 HomoloGene: 129366 GeneCards: CTSL
Gene location (Human)
Chromosome 9 (human)
Chr.Chromosome 9 (human)[1]
Chromosome 9 (human)
Genomic location for CTSL
Genomic location for CTSL
Band9q21.33Start87,724,051 bp[1]
End87,731,469 bp[1]
RNA expression pattern
Bgee
HumanMouse (ortholog)
Top expressed in
  • stromal cell of endometrium

  • islet of Langerhans

  • pericardium

  • gallbladder

  • upper lobe of left lung

  • left uterine tube

  • renal medulla

  • gastric mucosa

  • smooth muscle tissue

  • Achilles tendon
    n/a
More reference expression data
BioGPS
More reference expression data
Gene ontology
Molecular function
  • fibronectin binding
  • cysteine-type peptidase activity
  • collagen binding
  • histone binding
  • serpin family protein binding
  • peptidase activity
  • protein binding
  • cysteine-type endopeptidase activity
  • hydrolase activity
  • proteoglycan binding
  • serine-type endopeptidase activity
Cellular component
  • extracellular region
  • endolysosome lumen
  • lysosomal lumen
  • lysosome
  • extracellular exosome
  • nucleus
  • extracellular space
  • collagen-containing extracellular matrix
  • multivesicular body
  • plasma membrane
Biological process
  • macrophage apoptotic process
  • antigen processing and presentation
  • adaptive immune response
  • antigen processing and presentation of exogenous peptide antigen via MHC class II
  • extracellular matrix disassembly
  • proteolysis
  • toll-like receptor signaling pathway
  • cellular response to thyroid hormone stimulus
  • collagen catabolic process
  • proteolysis involved in cellular protein catabolic process
  • regulation of keratinocyte differentiation
Sources:Amigo / QuickGO
Orthologs
SpeciesHumanMouse
Entrez

1514

n/a

Ensembl

ENSG00000135047

n/a

UniProt

P07711
Q9HBQ7

n/a

RefSeq (mRNA)
NM_001257971
NM_001257972
NM_001257973
NM_001912
NM_145918

NM_001382757
NM_001382758
NM_001382766
NM_001382767
NM_001382768

n/a

RefSeq (protein)
NP_001244900
NP_001244901
NP_001244902
NP_001903
NP_666023

NP_001369686
NP_001369687
NP_001369695
NP_001369696
NP_001369697
NP_001244902.1

n/a

Location (UCSC)Chr 9: 87.72 – 87.73 Mbn/a
PubMed search[2]n/a
Wikidata
View/Edit Human

Cathepsin L1 is a protein that in humans is encoded by the CTSL1 gene.[3][4][5] The protein is a cysteine cathepsin, a lysosomal cysteine protease that plays a major role in intracellular protein catabolism.[6][7][8][9]

Function

Cathepsin L1 is a member of the Peptidase C1 (cathepsin) MEROPS family, which plays an important role in diverse processes including normal lysosome mediated protein turnover, antigen and proprotein processing, and apoptosis.[10] Its substrates include collagen and elastin, as well as alpha-1 protease inhibitor, a major controlling element of neutrophil elastase activity. The encoded protein has been implicated in several pathologic processes, including myofibril necrosis in myopathies and in myocardial ischemia, and in the renal tubular response to proteinuria. This protein, which is a member of the peptidase C1 family, is a dimer composed of disulfide-linked heavy and light chains, both produced from a single protein precursor. At least two transcript variants encoding the same protein have been found for this gene.[5]

Viral entry

Cleavage of the SARS-CoV-2 S2 spike protein required for viral entry into cells can be accomplished by proteases TMPRSS2 located on the cell membrane, or by cathepsins (primarily cathepsin L) in endolysosomes.[11] Hydroxychloroquine inhibits the action of cathepsin L in endolysosomes, but because cathepsin L cleavage is minor compared to TMPRSS2 cleavage, hydroxychloroquine does little to inhibit SARS-CoV-2 infection.[11]

Inflammation

Although Cathepsin L is usually characterized as a lysosomal protease, it can be secreted, resulting in pathological inflammation.[12] Cathepsin L and other cysteine cathepsins tend to be secreted by macrophages and other tissue-invading immune cells when causing pathological inflammation.[13]

Interactions

CTSL1 has been shown to interact with Cystatin A.[14][15]

Distribution

Cathepsin L has been reported in many organisms including fish,[16] birds, mammals, and sponges.[17]

See also

References

  1. ^ a b c GRCh38: Ensembl release 89: ENSG00000135047 – Ensembl, May 2017
  2. ^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  3. ^ Chauhan SS, Popescu NC, Ray D, Fleischmann R, Gottesman MM, Troen BR (Feb 1993). "Cloning, genomic organization, and chromosomal localization of human cathepsin L". J Biol Chem. 268 (2): 1039–45. doi:10.1016/S0021-9258(18)54038-2. PMID 8419312.
  4. ^ Joseph LJ, Chang LC, Stamenkovich D, Sukhatme VP (Jun 1988). "Complete nucleotide and deduced amino acid sequences of human and murine preprocathepsin L. An abundant transcript induced by transformation of fibroblasts". J Clin Invest. 81 (5): 1621–9. doi:10.1172/JCI113497. PMC 442598. PMID 2835398.
  5. ^ a b "Entrez Gene: CTSL1 cathepsin L1".
  6. ^ Barrett AJ, Kirschke H (1981). "Cathepsin B, cathepsin H, and cathepsin L". Proteolytic Enzymes, Part C. Methods in Enzymology. Vol. 80 Pt C. pp. 535–561. doi:10.1016/s0076-6879(81)80043-2. ISBN 9780121819804. PMID 7043200.
  7. ^ Barrett AJ, Buttle DJ, Mason RW (1988). "Lysosomal cysteine proteinases". ISI Atlas of Science. Biochemistry. 1: 256–260.
  8. ^ Joseph LJ, Chang LC, Stamenkovich D, Sukhatme VP (May 1988). "Complete nucleotide and deduced amino acid sequences of human and murine preprocathepsin L. An abundant transcript induced by transformation of fibroblasts". The Journal of Clinical Investigation. 81 (5): 1621–1629. doi:10.1172/JCI113497. PMC 442598. PMID 2835398.
  9. ^ Kirschke H, Wikstrom P, Shaw E (February 1988). "Active center differences between cathepsins L and B: the S1 binding region". FEBS Letters. 228 (1): 128–130. doi:10.1016/0014-5793(88)80600-8. PMID 3342870.
  10. ^ Dickinson DP (2002). "Cysteine Peptidases of Mammals: Their Biological Roles and Potential Effects in the Oral Cavity and Other Tissues in Health and Disease". Critical Reviews in Oral Biology and Medicine. 13 (3): 238–75. doi:10.1177/154411130201300304. PMID 12090464.
  11. ^ a b Jackson CB, Farzan M, Chen B, Choe H (January 2022). "Mechanisms of SARS-CoV-2 entry into cells". Nature Reviews. Molecular Cell Biology. 23 (1): 3–20. doi:10.1038/s41580-021-00418-x. PMC 8491763. PMID 34611326.
  12. ^ Gomes CP, Fernandes DE, Casimiro F, da Mata GF, Passos MT, Varela P, et al. (2022). "Cathepsin L in COVID-19: From Pharmacological Evidences to Genetics". Frontiers in Cellular and Infection Microbiology. 10: 589505. doi:10.3389/fcimb.2020.589505. PMC 7753008. PMID 33364201.
  13. ^ Berdowska I, Matusiewicz M (October 2021). "Cathepsin L, transmembrane peptidase/serine subfamily member 2/4, and other host proteases in COVID-19 pathogenesis - with impact on gastrointestinal tract". World Journal of Gastroenterology. 27 (39): 6590–6600. doi:10.3748/wjg.v27.i39.6590. PMC 8554394. PMID 34754154.
  14. ^ Majerle A, Jerala Roman (Sep 2003). "Protein inhibitors form complexes with procathepsin L and augment cleavage of the propeptide". Arch. Biochem. Biophys. 417 (1): 53–8. doi:10.1016/S0003-9861(03)00319-9. ISSN 0003-9861. PMID 12921779.
  15. ^ Estrada S, Nycander M, Hill N J, Craven C J, Waltho J P, Björk I (May 1998). "The role of Gly-4 of human cystatin A (stefin A) in the binding of target proteinases. Characterization by kinetic and equilibrium methods of the interactions of cystatin A Gly-4 mutants with papain, cathepsin B, and cathepsin L". Biochemistry. 37 (20): 7551–60. doi:10.1021/bi980026r. ISSN 0006-2960. PMID 9585570.
  16. ^ Venkatesh K, Prasanth B, Rajesh P, Annie JG, Mukesh P, Jesu A (2014). "A murrel cysteine protease, cathepsin L: bioinformatics characterization, gene expression and proteolytic activity". Biologia. 39 (3): 395–406. Bibcode:2014Biolg..69..395K. doi:10.2478/s11756-013-0326-8.
  17. ^ Sevenich L, Pennacchio LA, Peters C, Reinheckel T (July 2006). "Human cathepsin L rescues the neurodegeneration and lethality in cathepsin B/L double-deficient mice". Biological Chemistry. 387 (7): 885–91. doi:10.1515/BC.2006.112. PMID 16913838. S2CID 27739485.

Further reading

  • Smith CG, Smith MT, Besch NF, et al. (1980). "Effect of delta 9-tetrahydrocannabinol (THC) on female reproductive function". Advances in the Biosciences. 22–23: 449–67. doi:10.1016/b978-0-08-023759-6.50040-8. ISBN 9780080237596. PMID 116880.
  • Goretzki L, Schmitt M, Mann K, et al. (1992). "Effective activation of the proenzyme form of the urokinase-type plasminogen activator (pro-uPA) by the cysteine protease cathepsin L." FEBS Lett. 297 (1–2): 112–8. doi:10.1016/0014-5793(92)80339-I. PMID 1551416. S2CID 45421148.
  • Dunn AD, Crutchfield HE, Dunn JT (1991). "Thyroglobulin processing by thyroidal proteases. Major sites of cleavage by cathepsins B, D, and L." J. Biol. Chem. 266 (30): 20198–204. doi:10.1016/S0021-9258(18)54909-7. PMID 1939080.
  • Stearns NA, Dong JM, Pan JX, et al. (1991). "Comparison of cathepsin L synthesized by normal and transformed cells at the gene, message, protein, and oligosaccharide levels". Arch. Biochem. Biophys. 283 (2): 447–57. doi:10.1016/0003-9861(90)90666-M. PMID 2275556.
  • Ritonja A, Popović T, Kotnik M, et al. (1988). "Amino acid sequences of the human kidney cathepsins H and L." FEBS Lett. 228 (2): 341–5. doi:10.1016/0014-5793(88)80028-0. PMID 3342889. S2CID 45768546.
  • Gal S, Gottesman MM (1988). "Isolation and sequence of a cDNA for human pro-(cathepsin L)". Biochem. J. 253 (1): 303–6. doi:10.1042/bj2530303. PMC 1149292. PMID 3421948.
  • Johnson DA, Barrett AJ, Mason RW (1986). "Cathepsin L inactivates alpha 1-proteinase inhibitor by cleavage in the reactive site region". J. Biol. Chem. 261 (31): 14748–51. doi:10.1016/S0021-9258(18)66935-2. PMID 3490478.
  • Mason RW, Walker JE, Northrop FD (1987). "The N-terminal amino acid sequences of the heavy and light chains of human cathepsin L. Relationship to a cDNA clone for a major cysteine proteinase from a mouse macrophage cell line". Biochem. J. 240 (2): 373–7. doi:10.1042/bj2400373. PMC 1147428. PMID 3545185.
  • Joseph L, Lapid S, Sukhatme V (1987). "The major ras induced protein in NIH3T3 cells is cathepsin L." Nucleic Acids Res. 15 (7): 3186. doi:10.1093/nar/15.7.3186. PMC 340927. PMID 3550705.
  • Kärgel HJ, Dettmer R, Etzold G, et al. (1982). "Action of rat liver cathepsin L on glucagon". Acta Biol. Med. Ger. 40 (9): 1139–43. PMID 7340337.
  • Bevec T, Stoka V, Pungercic G, et al. (1996). "Major histocompatibility complex class II-associated p41 invariant chain fragment is a strong inhibitor of lysosomal cathepsin L." J. Exp. Med. 183 (4): 1331–8. doi:10.1084/jem.183.4.1331. PMC 2192513. PMID 8666891.
  • Coulombe R, Grochulski P, Sivaraman J, et al. (1996). "Structure of human procathepsin L reveals the molecular basis of inhibition by the prosegment". EMBO J. 15 (20): 5492–503. doi:10.1002/j.1460-2075.1996.tb00934.x. PMC 452294. PMID 8896443.
  • Baumgrass R, Williamson MK, Price PA (1997). "Identification of peptide fragments generated by digestion of bovine and human osteocalcin with the lysosomal proteinases cathepsin B, D, L, H, and S." J. Bone Miner. Res. 12 (3): 447–55. doi:10.1359/jbmr.1997.12.3.447. PMID 9076588. S2CID 20815411.
  • Fujishima A, Imai Y, Nomura T, et al. (1997). "The crystal structure of human cathepsin L complexed with E-64". FEBS Lett. 407 (1): 47–50. doi:10.1016/S0014-5793(97)00216-0. PMID 9141479. S2CID 46288832.
  • Ménard R, Carmona E, Takebe S, et al. (1998). "Autocatalytic processing of recombinant human procathepsin L. Contribution of both intermolecular and unimolecular events in the processing of procathepsin L in vitro". J. Biol. Chem. 273 (8): 4478–84. doi:10.1074/jbc.273.8.4478. PMID 9468501.
  • Schick C, Pemberton PA, Shi GP, et al. (1998). "Cross-class inhibition of the cysteine proteinases cathepsins K, L, and S by the serpin squamous cell carcinoma antigen 1: a kinetic analysis". Biochemistry. 37 (15): 5258–66. doi:10.1021/bi972521d. PMID 9548757.
  • Estrada S, Nycander M, Hill NJ, et al. (1998). "The role of Gly-4 of human cystatin A (stefin A) in the binding of target proteinases. Characterization by kinetic and equilibrium methods of the interactions of cystatin A Gly-4 mutants with papain, cathepsin B, and cathepsin L.". Biochemistry. 37 (20): 7551–60. doi:10.1021/bi980026r. PMID 9585570.
  • Halfon S, Ford J, Foster J, et al. (1998). "Leukocystatin, a new Class II cystatin expressed selectively by hematopoietic cells". J. Biol. Chem. 273 (26): 16400–8. doi:10.1074/jbc.273.26.16400. PMID 9632704.

External links

  • The MEROPS online database for peptidases and their inhibitors: C01.032[permanent dead link]
  • Cathepsin+L at the U.S. National Library of Medicine Medical Subject Headings (MeSH)
  • v
  • t
  • e
  • 1cjl: CRYSTAL STRUCTURE OF A CYSTEINE PROTEASE PROFORM
    1cjl: CRYSTAL STRUCTURE OF A CYSTEINE PROTEASE PROFORM
  • 1cs8: CRYSTAL STRUCTURE OF PROCATHEPSIN L
    1cs8: CRYSTAL STRUCTURE OF PROCATHEPSIN L
  • 1icf: CRYSTAL STRUCTURE OF MHC CLASS II ASSOCIATED P41 II FRAGMENT IN COMPLEX WITH CATHEPSIN L
    1icf: CRYSTAL STRUCTURE OF MHC CLASS II ASSOCIATED P41 II FRAGMENT IN COMPLEX WITH CATHEPSIN L
  • 1mhw: Design of non-covalent inhibitors of human cathepsin L. From the 96-residue proregion to optimized tripeptides
    1mhw: Design of non-covalent inhibitors of human cathepsin L. From the 96-residue proregion to optimized tripeptides
Portal:
  • icon Biology


Stub icon

This article on a gene on human chromosome 9 is a stub. You can help Wikipedia by expanding it.

  • v
  • t
  • e