DAPK1

Protein-coding gene in the species Homo sapiens
DAPK1
Available structures
PDBOrtholog search: PDBe RCSB
List of PDB id codes

1IG1, 1JKK, 1JKL, 1JKS, 1JKT, 1P4F, 1WVX, 1WVY, 1YR5, 2W4J, 2W4K, 2X0G, 2XUU, 2XZS, 2Y0A, 2Y4P, 2Y4V, 2YAK, 3DFC, 3DGK, 3EH9, 3EHA, 3F5G, 3F5U, 3GU4, 3GU5, 3GU6, 3GU7, 3GU8, 3GUB, 3ZXT, 4B4L, 4PF4, 4TL0, 4TXC, 4UV0, 4YO4, 4YPD, 5AUW, 5AUU, 5AV1, 5AUY, 5AUT, 5AUX, 5AUV, 5AUZ, 5AV0, 5AV4, 5AV2, 5AV3

Identifiers
AliasesDAPK1, DAPK, death associated protein kinase 1, ROCO3
External IDsOMIM: 600831 MGI: 1916885 HomoloGene: 3626 GeneCards: DAPK1
Gene location (Human)
Chromosome 9 (human)
Chr.Chromosome 9 (human)[1]
Chromosome 9 (human)
Genomic location for DAPK1
Genomic location for DAPK1
Band9q21.33Start87,497,228 bp[1]
End87,708,634 bp[1]
Gene location (Mouse)
Chromosome 13 (mouse)
Chr.Chromosome 13 (mouse)[2]
Chromosome 13 (mouse)
Genomic location for DAPK1
Genomic location for DAPK1
Band13 B2|13 32.53 cMStart60,749,761 bp[2]
End60,911,005 bp[2]
RNA expression pattern
Bgee
HumanMouse (ortholog)
Top expressed in
  • germinal epithelium

  • palpebral conjunctiva

  • placenta

  • middle temporal gyrus

  • parietal pleura

  • corpus epididymis

  • gallbladder

  • pancreatic ductal cell

  • monocyte

  • metanephros
Top expressed in
  • saccule

  • left lung lobe

  • olfactory tubercle

  • subiculum

  • piriform cortex

  • pineal gland

  • habenula

  • right lung lobe

  • nucleus accumbens

  • cingulate gyrus
More reference expression data
BioGPS


More reference expression data
Gene ontology
Molecular function
  • transferase activity
  • nucleotide binding
  • GTP binding
  • syntaxin-1 binding
  • kinase activity
  • protein binding
  • identical protein binding
  • calmodulin-dependent protein kinase activity
  • protein serine/threonine kinase activity
  • calmodulin binding
  • ATP binding
  • protein kinase activity
Cellular component
  • plasma membrane
  • actin cytoskeleton
  • cytoskeleton
  • cytoplasm
  • nucleus
Biological process
  • regulation of apoptotic process
  • negative regulation of translation
  • phosphorylation
  • negative regulation of apoptotic process
  • positive regulation of cysteine-type endopeptidase activity involved in apoptotic process
  • regulation of autophagy
  • negative regulation of extrinsic apoptotic signaling pathway via death domain receptors
  • apoptotic signaling pathway
  • protein autophosphorylation
  • regulation of translation
  • extrinsic apoptotic signaling pathway via death domain receptors
  • signal transduction
  • apoptotic process
  • regulation of NMDA receptor activity
  • protein phosphorylation
  • positive regulation of autophagy
  • intracellular signal transduction
  • cellular response to interferon-gamma
  • cellular response to hydroperoxide
Sources:Amigo / QuickGO
Orthologs
SpeciesHumanMouse
Entrez

1612

69635

Ensembl

ENSG00000196730

ENSMUSG00000021559

UniProt

P53355

Q80YE7

RefSeq (mRNA)

NM_001288729
NM_001288730
NM_001288731
NM_004938

NM_001285917
NM_029653
NM_134062

RefSeq (protein)

NP_001275658
NP_001275659
NP_001275660
NP_004929

NP_001272846
NP_083929
NP_598823

Location (UCSC)Chr 9: 87.5 – 87.71 MbChr 13: 60.75 – 60.91 Mb
PubMed search[3][4]
Wikidata
View/Edit HumanView/Edit Mouse

Death-associated protein kinase 1 is an enzyme that in humans is encoded by the DAPK1 gene.[5]

Function

Death-associated protein kinase 1 is a positive mediator of gamma-interferon induced programmed cell death. DAPK1 encodes a structurally unique 160-kD calmodulin dependent serine-threonine kinase that carries 8 ankyrin repeats and 2 putative P-loop consensus sites. It is a tumor suppressor candidate.[6]

In melanocytic cells DAPK1 gene expression may be regulated by MITF.[7]

As a drug target

Depletion of DAPK1 results in inhibition of tumor cell count and volume growth in cellular and animal models of triple receptor-negative breast cancer, from individuals with p53-mutant cancers.[8] This has not been demonstrated in actual patients.

References

  1. ^ a b c GRCh38: Ensembl release 89: ENSG00000196730 – Ensembl, May 2017
  2. ^ a b c GRCm38: Ensembl release 89: ENSMUSG00000021559 – Ensembl, May 2017
  3. ^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  4. ^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  5. ^ Feinstein E, Druck T, Kastury K, Berissi H, Goodart SA, Overhauser J, Kimchi A, Huebner K (Sep 1995). "Assignment of DAP1 and DAPK--genes that positively mediate programmed cell death triggered by IFN-gamma--to chromosome regions 5p12.2 and 9q34.1, respectively". Genomics. 29 (1): 305–7. doi:10.1006/geno.1995.1255. PMID 8530096.
  6. ^ "Entrez Gene: DAPK1 death-associated protein kinase 1".
  7. ^ Hoek KS, Schlegel NC, Eichhoff OM, Widmer DS, Praetorius C, Einarsson SO, Valgeirsdottir S, Bergsteinsdottir K, Schepsky A, Dummer R, Steingrimsson E (Dec 2008). "Novel MITF targets identified using a two-step DNA microarray strategy". Pigment Cell & Melanoma Research. 21 (6): 665–76. doi:10.1111/j.1755-148X.2008.00505.x. PMID 19067971. S2CID 24698373.
  8. ^ Zhao J, Zhao D, Poage GM, Mazumdar A, Zhang Y, Hill JL, Hartman ZC, Savage MI, Mills GB, Brown PH (Jul 2015). "Death-associated protein kinase 1 promotes growth of p53-mutant cancers". The Journal of Clinical Investigation. 125 (7): 2707–20. doi:10.1172/JCI70805. PMC 4563671. PMID 26075823.

Further reading

  • Deiss LP, Feinstein E, Berissi H, Cohen O, Kimchi A (Jan 1995). "Identification of a novel serine/threonine kinase and a novel 15-kD protein as potential mediators of the gamma interferon-induced cell death". Genes & Development. 9 (1): 15–30. doi:10.1101/gad.9.1.15. PMID 7828849.
  • Inbal B, Shani G, Cohen O, Kissil JL, Kimchi A (Feb 2000). "Death-associated protein kinase-related protein 1, a novel serine/threonine kinase involved in apoptosis". Molecular and Cellular Biology. 20 (3): 1044–54. doi:10.1128/MCB.20.3.1044-1054.2000. PMC 85221. PMID 10629061.
  • Jin Y, Blue EK, Dixon S, Hou L, Wysolmerski RB, Gallagher PJ (Oct 2001). "Identification of a new form of death-associated protein kinase that promotes cell survival". The Journal of Biological Chemistry. 276 (43): 39667–78. doi:10.1074/jbc.M101886200. PMC 2823794. PMID 11485996.
  • Shohat G, Spivak-Kroizman T, Cohen O, Bialik S, Shani G, Berrisi H, Eisenstein M, Kimchi A (Dec 2001). "The pro-apoptotic function of death-associated protein kinase is controlled by a unique inhibitory autophosphorylation-based mechanism". The Journal of Biological Chemistry. 276 (50): 47460–7. doi:10.1074/jbc.M105133200. PMID 11579085.
  • Soria JC, Rodriguez M, Liu DD, Lee JJ, Hong WK, Mao L (Jan 2002). "Aberrant promoter methylation of multiple genes in bronchial brush samples from former cigarette smokers". Cancer Research. 62 (2): 351–5. PMID 11809677.
  • Larramendy ML, Niini T, Elonen E, Nagy B, Ollila J, Vihinen M, Knuutila S (Jun 2002). "Overexpression of translocation-associated fusion genes of FGFRI, MYC, NPMI, and DEK, but absence of the translocations in acute myeloid leukemia. A microarray analysis". Haematologica. 87 (6): 569–77. PMID 12031912.
  • Hasegawa M, Nelson HH, Peters E, Ringstrom E, Posner M, Kelsey KT (Jun 2002). "Patterns of gene promoter methylation in squamous cell cancer of the head and neck". Oncogene. 21 (27): 4231–6. doi:10.1038/sj.onc.1205528. PMID 12082610. S2CID 24566542.
  • Jin Y, Blue EK, Dixon S, Shao Z, Gallagher PJ (Dec 2002). "A death-associated protein kinase (DAPK)-interacting protein, DIP-1, is an E3 ubiquitin ligase that promotes tumor necrosis factor-induced apoptosis and regulates the cellular levels of DAPK". The Journal of Biological Chemistry. 277 (49): 46980–6. doi:10.1074/jbc.M208585200. PMC 2824503. PMID 12351649.
  • Wang WJ, Kuo JC, Yao CC, Chen RH (Oct 2002). "DAP-kinase induces apoptosis by suppressing integrin activity and disrupting matrix survival signals". The Journal of Cell Biology. 159 (1): 169–79. doi:10.1083/jcb.200204050. PMC 2173490. PMID 12370243.
  • Nakatsuka S, Takakuwa T, Tomita Y, Hoshida Y, Nishiu M, Yamaguchi M, Nishii K, Yang WI, Aozasa K (Jan 2003). "Hypermethylation of death-associated protein (DAP) kinase CpG island is frequent not only in B-cell but also in T- and natural killer (NK)/T-cell malignancies". Cancer Science. 94 (1): 87–91. doi:10.1111/j.1349-7006.2003.tb01357.x. PMID 12708480. S2CID 11818957.
  • Tian JH, Das S, Sheng ZH (Jul 2003). "Ca2+-dependent phosphorylation of syntaxin-1A by the death-associated protein (DAP) kinase regulates its interaction with Munc18". The Journal of Biological Chemistry. 278 (28): 26265–74. doi:10.1074/jbc.M300492200. PMID 12730201.
  • Gonzalez-Gomez P, Bello MJ, Alonso ME, Lomas J, Arjona D, Amiñoso C, De Campos JM, Isla A, Gutierrez M, Rey JA (2004). "Frequent death-associated protein-kinase promoter hypermethylation in brain metastases of solid tumors". Oncology Reports. 10 (4): 1031–3. doi:10.3892/or.10.4.1031. PMID 12792765.
  • Matsumoto H, Nagao M, Ogawa S, Kanehiro H, Hisanaga M, Ko S, Ikeda N, Fujii H, Koyama F, Mukogawa T, Nakajima Y (2003). "Prognostic significance of death-associated protein-kinase expression in hepatocellular carcinomas". Anticancer Research. 23 (2B): 1333–41. PMID 12820391.
  • Henshall DC, Araki T, Schindler CK, Shinoda S, Lan JQ, Simon RP (Sep 2003). "Expression of death-associated protein kinase and recruitment to the tumor necrosis factor signaling pathway following brief seizures". Journal of Neurochemistry. 86 (5): 1260–70. doi:10.1046/j.1471-4159.2003.01934.x. PMID 12911633. S2CID 21971958.
  • Voso MT, Scardocci A, Guidi F, Zini G, Di Mario A, Pagano L, Hohaus S, Leone G (Jan 2004). "Aberrant methylation of DAP-kinase in therapy-related acute myeloid leukemia and myelodysplastic syndromes". Blood. 103 (2): 698–700. doi:10.1182/blood-2003-07-2249. PMID 14504087.
  • Jin Y, Gallagher PJ (Dec 2003). "Antisense depletion of death-associated protein kinase promotes apoptosis". The Journal of Biological Chemistry. 278 (51): 51587–93. doi:10.1074/jbc.M309165200. PMC 2823796. PMID 14530257.
  • Kim WS, Son HJ, Park JO, Song SY, Park C (Dec 2003). "Promoter methylation and down-regulation of DAPK is associated with gastric atrophy". International Journal of Molecular Medicine. 12 (6): 827–30. doi:10.3892/ijmm.12.6.827. PMID 14612952.
  • Bai T, Tanaka T, Yukawa K, Maeda M, Umesaki N (Mar 2004). "Reduced expression of death-associated protein kinase in human uterine and ovarian carcinoma cells". Oncology Reports. 11 (3): 661–5. doi:10.3892/or.11.3.661. PMID 14767518.
  • v
  • t
  • e
  • 1ig1: 1.8A X-Ray structure of ternary complex of a catalytic domain of death-associated protein kinase with ATP analogue and Mn.
    1ig1: 1.8A X-Ray structure of ternary complex of a catalytic domain of death-associated protein kinase with ATP analogue and Mn.
  • 1jkk: 2.4A X-RAY STRUCTURE OF TERNARY COMPLEX OF A CATALYTIC DOMAIN OF DEATH-ASSOCIATED PROTEIN KINASE WITH ATP ANALOGUE AND MG.
    1jkk: 2.4A X-RAY STRUCTURE OF TERNARY COMPLEX OF A CATALYTIC DOMAIN OF DEATH-ASSOCIATED PROTEIN KINASE WITH ATP ANALOGUE AND MG.
  • 1jkl: 1.6A X-RAY STRUCTURE OF BINARY COMPLEX OF A CATALYTIC DOMAIN OF DEATH-ASSOCIATED PROTEIN KINASE WITH ATP ANALOGUE
    1jkl: 1.6A X-RAY STRUCTURE OF BINARY COMPLEX OF A CATALYTIC DOMAIN OF DEATH-ASSOCIATED PROTEIN KINASE WITH ATP ANALOGUE
  • 1jks: 1.5A X-RAY STRUCTURE OF APO FORM OF A CATALYTIC DOMAIN OF DEATH-ASSOCIATED PROTEIN KINASE
    1jks: 1.5A X-RAY STRUCTURE OF APO FORM OF A CATALYTIC DOMAIN OF DEATH-ASSOCIATED PROTEIN KINASE
  • 1jkt: TETRAGONAL CRYSTAL FORM OF A CATALYTIC DOMAIN OF DEATH-ASSOCIATED PROTEIN KINASE
    1jkt: TETRAGONAL CRYSTAL FORM OF A CATALYTIC DOMAIN OF DEATH-ASSOCIATED PROTEIN KINASE
  • 1p4f: DEATH ASSOCIATED PROTEIN KINASE CATALYTIC DOMAIN WITH BOUND INHIBITOR FRAGMENT
    1p4f: DEATH ASSOCIATED PROTEIN KINASE CATALYTIC DOMAIN WITH BOUND INHIBITOR FRAGMENT
  • 1wvw: Crystal structures of kinase domain of DAP kinase in complex with small molecular inhibitors
    1wvw: Crystal structures of kinase domain of DAP kinase in complex with small molecular inhibitors
  • 1wvx: Crystal structures of kinase domain of DAP kinase in complex with small molecular inhibitors
    1wvx: Crystal structures of kinase domain of DAP kinase in complex with small molecular inhibitors
  • 1wvy: Crystal structures of kinase domain of DAP kinase in complex with small molecular inhibitors
    1wvy: Crystal structures of kinase domain of DAP kinase in complex with small molecular inhibitors
  • v
  • t
  • e
Non-specific serine/threonine protein kinases (EC 2.7.11.1)
Pyruvate dehydrogenase kinase (EC 2.7.11.2)
Dephospho-(reductase kinase) kinase (EC 2.7.11.3)
3-methyl-2-oxobutanoate dehydrogenase (acetyl-transferring) kinase (EC 2.7.11.4)
(isocitrate dehydrogenase (NADP+)) kinase (EC 2.7.11.5)
(tyrosine 3-monooxygenase) kinase (EC 2.7.11.6)
Myosin-heavy-chain kinase (EC 2.7.11.7)
Fas-activated serine/threonine kinase (EC 2.7.11.8)
Goodpasture-antigen-binding protein kinase (EC 2.7.11.9)
  • -
IκB kinase (EC 2.7.11.10)
cAMP-dependent protein kinase (EC 2.7.11.11)
cGMP-dependent protein kinase (EC 2.7.11.12)
Protein kinase C (EC 2.7.11.13)
Rhodopsin kinase (EC 2.7.11.14)
Beta adrenergic receptor kinase (EC 2.7.11.15)
G-protein coupled receptor kinases (EC 2.7.11.16)
Ca2+/calmodulin-dependent (EC 2.7.11.17)
Myosin light-chain kinase (EC 2.7.11.18)
Phosphorylase kinase (EC 2.7.11.19)
Elongation factor 2 kinase (EC 2.7.11.20)
Polo kinase (EC 2.7.11.21)
Serine/threonine-specific protein kinases (EC 2.7.11.21-EC 2.7.11.30)
Polo kinase (EC 2.7.11.21)
Cyclin-dependent kinase (EC 2.7.11.22)
(RNA-polymerase)-subunit kinase (EC 2.7.11.23)
Mitogen-activated protein kinase (EC 2.7.11.24)
MAP3K (EC 2.7.11.25)
Tau-protein kinase (EC 2.7.11.26)
(acetyl-CoA carboxylase) kinase (EC 2.7.11.27)
  • -
Tropomyosin kinase (EC 2.7.11.28)
  • -
Low-density-lipoprotein receptor kinase (EC 2.7.11.29)
  • -
Receptor protein serine/threonine kinase (EC 2.7.11.30)
MAP2K
Portal:
  • icon Biology


Stub icon

This article on a gene on human chromosome 9 is a stub. You can help Wikipedia by expanding it.

  • v
  • t
  • e