NFYA

Protein-coding gene in the species Homo sapiens

NFYA
Available structures
PDBOrtholog search: PDBe RCSB
List of PDB id codes

4AWL

Identifiers
AliasesNFYA, CBF-A, CBF-B, HAP2, NF-YA, nuclear transcription factor Y subunit alpha
External IDsOMIM: 189903 MGI: 97316 HomoloGene: 32114 GeneCards: NFYA
Gene location (Human)
Chromosome 6 (human)
Chr.Chromosome 6 (human)[1]
Chromosome 6 (human)
Genomic location for NFYA
Genomic location for NFYA
Band6p21.1Start41,072,974 bp[1]
End41,102,403 bp[1]
Gene location (Mouse)
Chromosome 17 (mouse)
Chr.Chromosome 17 (mouse)[2]
Chromosome 17 (mouse)
Genomic location for NFYA
Genomic location for NFYA
Band17 C|17 23.99 cMStart48,693,913 bp[2]
End48,716,934 bp[2]
RNA expression pattern
Bgee
HumanMouse (ortholog)
Top expressed in
  • ganglionic eminence

  • sperm

  • secondary oocyte

  • bone marrow cells

  • islet of Langerhans

  • monocyte

  • stromal cell of endometrium

  • middle temporal gyrus

  • rectum

  • blood
Top expressed in
  • secondary oocyte

  • ganglionic eminence

  • thymus

  • yolk sac

  • placenta

  • bone marrow

  • ovary

  • uterus

  • spleen

  • lens
More reference expression data
BioGPS




More reference expression data
Gene ontology
Molecular function
  • DNA-binding transcription factor activity
  • DNA binding
  • core promoter sequence-specific DNA binding
  • protein binding
  • RNA polymerase II cis-regulatory region sequence-specific DNA binding
  • DNA-binding transcription factor activity, RNA polymerase II-specific
Cellular component
  • CCAAT-binding factor complex
  • protein-DNA complex
  • nucleus
  • nucleoplasm
  • RNA polymerase II transcription regulator complex
Biological process
  • positive regulation of transcription, DNA-templated
  • regulation of transcription, DNA-templated
  • transcription by RNA polymerase II
  • transcription, DNA-templated
  • rhythmic process
  • regulation of cholesterol biosynthetic process
  • regulation of transcription by RNA polymerase II
Sources:Amigo / QuickGO
Orthologs
SpeciesHumanMouse
Entrez

4800

18044

Ensembl

ENSG00000001167

ENSMUSG00000023994

UniProt

P23511

P23708

RefSeq (mRNA)

NM_021705
NM_002505

NM_001110832
NM_010913
NM_001347401
NM_001347402
NM_001374803

RefSeq (protein)

NP_002496
NP_068351

NP_001104302
NP_001334330
NP_001334331
NP_035043
NP_001361732

Location (UCSC)Chr 6: 41.07 – 41.1 MbChr 17: 48.69 – 48.72 Mb
PubMed search[3][4]
Wikidata
View/Edit HumanView/Edit Mouse

Nuclear transcription factor Y subunit alpha is a protein that in humans is encoded by the NFYA gene.[5][6]

Function

The protein encoded by this gene is one subunit of a trimeric complex NF-Y, forming a highly conserved transcription factor that binds to CCAAT motifs in the promoter regions in a variety of genes.[7] Subunit NFYA associates with a tight dimer composed of the NFYB and NFYC subunits, resulting in a trimer that binds to DNA with high specificity and affinity. The sequence specific interactions of the complex are made by the NFYA subunit, suggesting a role as the regulatory subunit. In addition, there is evidence of post-transcriptional regulation in this gene product, either by protein degradation or control of translation. Further regulation is represented by alternative splicing in the glutamine-rich activation domain, with clear tissue-specific preferences for the two isoforms.[8]

NF-Y complex serves as a pioneer factor by promoting chromatin accessibility to facilitate other co-localizing cell type-specific transcription factors.[9]

NF-Y has also been implicated as a central player in transcription start site (TSS) selection in animals.[10] It safeguards the integrity of the nucleosome-depleted region and PIC localization at protein-coding gene promoters.

Interactions

NFYA has been shown to interact with Serum response factor[11] and ZHX1.[11][12] NFYA, NFYB and NFYC form the NFY complex and it has been shown that the NFY complex serves as a pioneer factor by promoting chromatin accessibility to facilitate other co-localizing cell type-specific transcription factors.[7]

Structure

The atomic structure of the NFY heterotrimer in complex with dsDNA was resolved via X-ray crystallography (PDB ID 4awl).[13] Using one of the NFYA alpha helices as a template, structure inspired stapled peptides were designed to disrupt the NFY heterotrimer formation by preventing NFYA from binding to the NFYB/C heterodimer.[14]

References

  1. ^ a b c GRCh38: Ensembl release 89: ENSG00000001167 – Ensembl, May 2017
  2. ^ a b c GRCm38: Ensembl release 89: ENSMUSG00000023994 – Ensembl, May 2017
  3. ^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  4. ^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  5. ^ Li XY, Mattei MG, Zaleska-Rutczynska Z, et al. (November 1991). "One subunit of the transcription factor NF-Y maps close to the major histocompatibility complex in murine and human chromosomes". Genomics. 11 (3): 630–4. doi:10.1016/0888-7543(91)90070-U. PMID 1774067.
  6. ^ Maity SN, de Crombrugghe B (May 1998). "Role of the CCAAT-binding protein CBF/NF-Y in transcription". Trends in Biochemical Sciences. 23 (5): 174–8. doi:10.1016/S0968-0004(98)01201-8. PMID 9612081.
  7. ^ a b Oldfield AJ, Yang P, Conway AE, et al. (September 2014). "Histone-fold domain protein NF-Y promotes chromatin accessibility for cell type-specific master transcription factors". Molecular Cell. 55 (5): 708–22. doi:10.1016/j.molcel.2014.07.005. PMC 4157648. PMID 25132174.
  8. ^ "Entrez Gene: NFYA nuclear transcription factor Y, alpha".
  9. ^ Oldfield AJ, Yang P, Conway AE, et al. (September 2014). "Histone-fold domain protein NF-Y promotes chromatin accessibility for cell type-specific master transcription factors". Molecular Cell. 55 (5): 708–22. doi:10.1016/j.molcel.2014.07.005. PMC 4157648. PMID 25132174. [verification needed]
  10. ^ Oldfield AJ, Henriques T, Burkholder AB, et al. (11 July 2019). "NF-Y controls fidelity of transcription initiation at gene promoters through maintenance of the nucleosome-depleted region". Nature Communications. 10 (1): 3072. Bibcode:2019NatCo..10.3072O. doi:10.1038/s41467-019-10905-7. PMC 6624317. PMID 31296853.
  11. ^ a b Yamada K, Osawa H, Granner DK (October 1999). "Identification of proteins that interact with NF-YA". FEBS Letters. 460 (1): 41–5. doi:10.1016/S0014-5793(99)01311-3. PMID 10571058. S2CID 28576187.
  12. ^ Yamada K, Printz RL, Osawa H, et al. (August 1999). "Human ZHX1: cloning, chromosomal location, and interaction with transcription factor NF-Y". Biochemical and Biophysical Research Communications. 261 (3): 614–21. doi:10.1006/bbrc.1999.1087. PMID 10441475.
  13. ^ Nardini M, Gnesutta N, Donati G, et al. (2013). "Sequence-Specific Transcription Factor NF-Y Displays Histone-like DNA Binding and H2B-like Ubiquitination". Cell. 152 (1–2): 132–143. doi:10.1016/j.cell.2012.11.047. hdl:2318/1590740. ISSN 0092-8674. PMID 23332751.
  14. ^ Jeganathan S, Wendt M, Kiehstaller S, et al. (November 2019). "Constrained Peptides with Fine-Tuned Flexibility Inhibit NF-Y Transcription Factor Assembly". Angewandte Chemie. 58 (48): 17351–17358. doi:10.1002/anie.201907901. PMC 6900064. PMID 31539186.

Further reading

  • Mantovani R (October 1999). "The molecular biology of the CCAAT-binding factor NF-Y". Gene. 239 (1): 15–27. doi:10.1016/S0378-1119(99)00368-6. PMID 10571030.
  • Li XY, Mantovani R, Hooft van Huijsduijnen R, et al. (March 1992). "Evolutionary variation of the CCAAT-binding transcription factor NF-Y". Nucleic Acids Research. 20 (5): 1087–91. doi:10.1093/nar/20.5.1087. PMC 312095. PMID 1549471.
  • Li XY, Hooft van Huijsduijnen R, Mantovani R, et al. (May 1992). "Intron-exon organization of the NF-Y genes. Tissue-specific splicing modifies an activation domain". The Journal of Biological Chemistry. 267 (13): 8984–90. doi:10.1016/S0021-9258(19)50377-5. PMID 1577736.
  • Becker DM, Fikes JD, Guarente L (March 1991). "A cDNA encoding a human CCAAT-binding protein cloned by functional complementation in yeast". Proceedings of the National Academy of Sciences of the United States of America. 88 (5): 1968–72. Bibcode:1991PNAS...88.1968B. doi:10.1073/pnas.88.5.1968. PMC 51147. PMID 2000400.
  • Vuorio T, Maity SN, de Crombrugghe B (December 1990). "Purification and molecular cloning of the "A" chain of a rat heteromeric CCAAT-binding protein. Sequence identity with the yeast HAP3 transcription factor". The Journal of Biological Chemistry. 265 (36): 22480–6. doi:10.1016/S0021-9258(18)45730-4. PMID 2266139.
  • Mantovani R, Li XY, Pessara U, et al. (August 1994). "Dominant negative analogs of NF-YA". The Journal of Biological Chemistry. 269 (32): 20340–6. doi:10.1016/S0021-9258(17)31997-X. PMID 8051128.
  • Currie RA (December 1997). "Functional interaction between the DNA binding subunit trimerization domain of NF-Y and the high mobility group protein HMG-I(Y)". The Journal of Biological Chemistry. 272 (49): 30880–8. doi:10.1074/jbc.272.49.30880. PMID 9388234.
  • Currie RA (July 1998). "Biochemical characterization of the NF-Y transcription factor complex during B lymphocyte development". The Journal of Biological Chemistry. 273 (29): 18220–9. doi:10.1074/jbc.273.29.18220. PMID 9660784.
  • Roder K, Wolf SS, Larkin KJ, et al. (June 1999). "Interaction between the two ubiquitously expressed transcription factors NF-Y and Sp1". Gene. 234 (1): 61–9. doi:10.1016/S0378-1119(99)00180-8. PMID 10393239.
  • Yamada K, Printz RL, Osawa H, et al. (August 1999). "Human ZHX1: cloning, chromosomal location, and interaction with transcription factor NF-Y". Biochemical and Biophysical Research Communications. 261 (3): 614–21. doi:10.1006/bbrc.1999.1087. PMID 10441475.
  • Yamada K, Osawa H, Granner DK (October 1999). "Identification of proteins that interact with NF-YA". FEBS Letters. 460 (1): 41–5. doi:10.1016/S0014-5793(99)01311-3. PMID 10571058. S2CID 28576187.
  • Fan W, Jin S, Tong T, et al. (March 2002). "BRCA1 regulates GADD45 through its interactions with the OCT-1 and CAAT motifs". The Journal of Biological Chemistry. 277 (10): 8061–7. doi:10.1074/jbc.M110225200. PMID 11777930.
  • Faniello MC, Chirico G, Quaresima B, et al. (April 2002). "An alternative model of H ferritin promoter transactivation by c-Jun". The Biochemical Journal. 363 (Pt 1): 53–8. doi:10.1042/0264-6021:3630053. PMC 1222450. PMID 11903046.
  • Bevilacqua MA, Faniello MC, Iovine B, et al. (November 2002). "Transcription factor NF-Y regulates differentiation of CaCo-2 cells". Archives of Biochemistry and Biophysics. 407 (1): 39–44. doi:10.1016/S0003-9861(02)00436-8. PMID 12392713.
  • Ge Y, Jensen TL, Matherly LH, et al. (December 2002). "Synergistic regulation of human cystathionine-beta-synthase-1b promoter by transcription factors NF-YA isoforms and Sp1". Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression. 1579 (2–3): 73–80. doi:10.1016/s0167-4781(02)00509-2. PMID 12427542.
  • Salsi V, Caretti G, Wasner M, et al. (February 2003). "Interactions between p300 and multiple NF-Y trimers govern cyclin B2 promoter function". The Journal of Biological Chemistry. 278 (9): 6642–50. doi:10.1074/jbc.M210065200. hdl:11380/1202119. PMID 12482752.
  • Peng Y, Jahroudi N (March 2003). "The NFY transcription factor inhibits von Willebrand factor promoter activation in non-endothelial cells through recruitment of histone deacetylases". The Journal of Biological Chemistry. 278 (10): 8385–94. doi:10.1074/jbc.M213156200. PMID 12511565.

External links

This article incorporates text from the United States National Library of Medicine, which is in the public domain.

  • v
  • t
  • e
(1) Basic domains
(1.1) Basic leucine zipper (bZIP)
(1.2) Basic helix-loop-helix (bHLH)
Group A
Group B
Group C
bHLH-PAS
Group D
Group E
Group F
bHLH-COE
(1.3) bHLH-ZIP
(1.4) NF-1
(1.5) RF-X
(1.6) Basic helix-span-helix (bHSH)
(2) Zinc finger DNA-binding domains
(2.1) Nuclear receptor (Cys4)
subfamily 1
subfamily 2
subfamily 3
subfamily 4
subfamily 5
subfamily 6
subfamily 0
(2.2) Other Cys4
(2.3) Cys2His2
(2.4) Cys6
(2.5) Alternating composition
(2.6) WRKY
(3) Helix-turn-helix domains
(3.1) Homeodomain
Antennapedia
ANTP class
protoHOX
Hox-like
metaHOX
NK-like
other
(3.2) Paired box
(3.3) Fork head / winged helix
(3.4) Heat shock factors
(3.5) Tryptophan clusters
(3.6) TEA domain
  • transcriptional enhancer factor
(4) β-Scaffold factors with minor groove contacts
(4.1) Rel homology region
(4.2) STAT
(4.3) p53-like
(4.4) MADS box
(4.6) TATA-binding proteins
(4.7) High-mobility group
(4.9) Grainyhead
(4.10) Cold-shock domain
(4.11) Runt
(0) Other transcription factors
(0.2) HMGI(Y)
(0.3) Pocket domain
(0.5) AP-2/EREBP-related factors
(0.6) Miscellaneous
see also transcription factor/coregulator deficiencies


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