TSPAN12

Protein-coding gene in humans

TSPAN12

Identifiers

Aliases

TSPAN12, EVR5, NET-2, NET2, TM4SF12, tetraspanin 12

External IDs

OMIM: 613138 MGI: 1889818 HomoloGene: 8212 GeneCards: TSPAN12

Gene location (Human)

Chr.	Chromosome 7 (human)^[1]

Band	7q31.31	Start	120,787,320 bp^[1]
Band	7q31.31	End	120,858,402 bp^[1]

Gene location (Mouse)

Chr.	Chromosome 6 (mouse)^[2]

Band	6\|6 A3.1	Start	21,771,394 bp^[2]
Band	6\|6 A3.1	End	21,852,514 bp^[2]

RNA expression pattern

Bgee

Human

Mouse (ortholog)

Top expressed in

oocyte

kidney tubule

secondary oocyte

right adrenal gland

right ventricle

lower lobe of lung

jejunal mucosa

glomerulus

metanephric glomerulus

left adrenal gland

Top expressed in

brown adipose tissue

parotid gland

submandibular gland

white adipose tissue

intercostal muscle

liver

adrenal gland

mucosa of urinary bladder

left lobe of liver

otic placode

More reference expression data

BioGPS

n/a

Gene ontology
Molecular function	protein binding Wnt-activated receptor activity
Cellular component	membrane plasma membrane integral component of membrane integral component of plasma membrane
Biological process	angiogenesis retina layer formation regulation of angiogenesis cell surface receptor signaling pathway Wnt signaling pathway
Sources:Amigo / QuickGO

Orthologs

Species

Human

Mouse

Entrez


23554


269831

Ensembl


ENSG00000106025


ENSMUSG00000029669

UniProt


O95859


Q8BKT6

RefSeq (mRNA)


NM_012338


NM_173007 NM_001363814

RefSeq (protein)


NP_036470


NP_766595 NP_001350743

Location (UCSC)

Chr 7: 120.79 – 120.86 Mb

Chr 6: 21.77 – 21.85 Mb

PubMed search

^[3]

^[4]

Wikidata

View/Edit Human

View/Edit Mouse

Tetraspanin-12 (Tspan-12) also known as tetraspan NET-2 (NET2) or transmembrane 4 superfamily member 12 (TM4SF12) is a tetraspanin protein that in humans is encoded by the TSPAN12 gene. Tetraspanin-12 is found in the membrane of a variety of cells. It has an unusually long C-terminal intracellular tail of approximately 60 amino acids.

Function

Its main binding partner is the ADAM10 protein,^[5] a sheddase that interacts with a variety of adhesion molecules that are found on the cell membrane including L1-CAM, E-Cadherin, N-Cadherin and CD44.^[6]^[7] It also binds to the MT1-MMP metalloprotease protein that is closely related to ADAM10 but has a minimal effect on promotion of expression and function.^[8] TSPAN12 also seems to regulate vascular development, as shown by a study involving TSPAN12 knockout mice.^[9] TSPAN12 is a significant contributor to primary and metastatic cancer and is responsible for protecting β-catenin from degradation.^[10]

References

^ ^a ^b ^c GRCh38: Ensembl release 89: ENSG00000106025 – Ensembl, May 2017
^ ^a ^b ^c GRCm38: Ensembl release 89: ENSMUSG00000029669 – Ensembl, May 2017
^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ Xu D, Sharma C, Hemler ME (November 2009). "Tetraspanin12 regulates ADAM10-dependent cleavage of amyloid precursor protein". FASEB J. 23 (11): 3674–81. doi:10.1096/fj.09-133462. PMC 2775005. PMID 19587294.
^ Lee SB, Schramme A, Doberstein K, Dummer R, Abdel-Bakky MS, Keller S, Altevogt P, Oh ST, Reichrath J, Oxmann D, Pfeilschifter J, Mihic-Probst D, Gutwein P (March 2010). "ADAM10 is upregulated in melanoma metastasis compared with primary melanoma". J. Invest. Dermatol. 130 (3): 763–73. doi:10.1038/jid.2009.335. PMID 19865098.
^ Anderegg U, Eichenberg T, Parthaune T, Haiduk C, Saalbach A, Milkova L, Ludwig A, Grosche J, Averbeck M, Gebhardt C, Voelcker V, Sleeman JP, Simon JC (June 2009). "ADAM10 is the constitutive functional sheddase of CD44 in human melanoma cells". J. Invest. Dermatol. 129 (6): 1471–82. doi:10.1038/jid.2008.323. PMID 18971959.
^ Lafleur MA, Xu D, Hemler ME (April 2009). "Tetraspanin proteins regulate membrane type-1 matrix metalloproteinase-dependent pericellular proteolysis". Mol. Biol. Cell. 20 (7): 2030–40. doi:10.1091/mbc.E08-11-1149. PMC 2663921. PMID 19211836.
^ Junge HJ, Yang S, Burton JB, Paes K, Shu X, French DM, Costa M, Rice DS, Ye W (October 2009). "TSPAN12 regulates retinal vascular development by promoting Norrin- but not Wnt-induced FZD4/beta-catenin signaling". Cell. 139 (2): 299–311. doi:10.1016/j.cell.2009.07.048. PMID 19837033. S2CID 2276711.
^ Knoblich K, Wang HX, Sharma C, Fletcher AL, Turley SJ, Hemler ME (April 2014). "Tetraspanin TSPAN12 regulates tumor growth and metastasis and inhibits β-catenin degradation". Cell Mol Life Sci. 71 (7): 1305–14. doi:10.1007/s00018-013-1444-8. PMID 23955570. S2CID 16135992.