Bone morphogenetic protein 4

BMP4
Identifiers
Aliases	BMP4, BMP2B, BMP2B1, MCOPS6, OFC11, ZYME, bone morphogenetic protein 4
External IDs	OMIM: 112262 MGI: 88180 HomoloGene: 7247 GeneCards: BMP4
Gene location (Mouse) Chr. Chromosome 14 (mouse)[1] Band 14 C1|14 23.95 cM Start 46,620,977 bp[1] End 46,628,126 bp[1]
RNA expression pattern Bgee Human Mouse (ortholog) n/a Top expressed in meninges semicircular canal surface ectoderm abdominal wall posterior semicircular canal amnion calvaria external carotid artery anterior semicircular canal vas deferens BioGPS More reference expression data
Gene ontology Molecular function heparin binding cytokine activity co-receptor binding transforming growth factor beta receptor binding growth factor activity BMP receptor binding protein binding chemoattractant activity Cellular component extracellular region extracellular space endoplasmic reticulum lumen Biological process embryonic skeletal system morphogenesis negative regulation of T cell differentiation in thymus germ cell development skeletal system development mesenchymal cell differentiation involved in renal system development cardiac septum development ureteric bud development positive regulation of protein phosphorylation renal system process positive regulation of endothelial cell differentiation negative regulation of immature T cell proliferation in thymus bud elongation involved in lung branching tendon cell differentiation anatomical structure formation involved in morphogenesis ureter epithelial cell differentiation negative regulation of cell cycle mesenchymal to epithelial transition involved in metanephros morphogenesis trachea development post-embryonic development monocyte differentiation specification of ureteric bud anterior/posterior symmetry by BMP signaling pathway blood vessel endothelial cell proliferation involved in sprouting angiogenesis BMP signaling pathway involved in renal system segmentation cranial suture morphogenesis mesonephros development odontogenesis of dentin-containing tooth telencephalon regionalization negative regulation of chondrocyte differentiation blood vessel development negative regulation of mitotic nuclear division angiogenesis prostate gland morphogenesis positive regulation of ERK1 and ERK2 cascade smooth muscle tissue development BMP signaling pathway involved in heart induction negative regulation of epithelial cell proliferation tissue development metanephric collecting duct development inner ear receptor cell differentiation mesodermal cell fate determination metanephros development type B pancreatic cell development regulation of pathway-restricted SMAD protein phosphorylation negative regulation of cell population proliferation steroid hormone mediated signaling pathway mammary gland formation positive regulation of collagen biosynthetic process renal system development negative regulation of myoblast differentiation cell fate commitment common-partner SMAD protein phosphorylation glomerular visceral epithelial cell development SMAD protein signal transduction ossification kidney development lung development ureter smooth muscle cell differentiation embryonic digit morphogenesis epithelial-mesenchymal cell signaling negative regulation of thymocyte apoptotic process mesenchymal cell differentiation involved in kidney development negative regulation of cell death regulation of odontogenesis of dentin-containing tooth BMP signaling pathway involved in ureter morphogenesis mesenchymal cell proliferation involved in ureteric bud development smooth muscle cell differentiation lymphoid progenitor cell differentiation epithelium development positive regulation of transcription, DNA-templated deltoid tuberosity development negative regulation of prostatic bud formation heart development telencephalon development branching involved in ureteric bud morphogenesis positive regulation of kidney development cartilage development embryonic limb morphogenesis negative regulation of MAP kinase activity positive regulation of cartilage development lens induction in camera-type eye positive regulation of neuron differentiation branching involved in prostate gland morphogenesis regulation of protein import into nucleus positive regulation of cell differentiation erythrocyte differentiation smoothened signaling pathway camera-type eye development secondary heart field specification negative regulation of phosphorylation regulation of smooth muscle cell differentiation regulation of cell fate commitment regulation of branching involved in prostate gland morphogenesis cell differentiation positive regulation of branching involved in lung morphogenesis chondrocyte differentiation regulation of cartilage development organ induction positive regulation of epithelial cell proliferation epithelial cell proliferation involved in lung morphogenesis negative regulation of cell proliferation involved in heart morphogenesis negative regulation of apoptotic process positive regulation of ossification endochondral ossification regulation of smooth muscle cell proliferation regulation of morphogenesis of a branching structure BMP signaling pathway macrophage differentiation negative regulation of metanephric comma-shaped body morphogenesis embryonic skeletal system development mesenchymal cell proliferation involved in ureter development osteoblast differentiation hematopoietic progenitor cell differentiation positive regulation of BMP signaling pathway regulation of gene expression embryonic cranial skeleton morphogenesis dorsal/ventral neural tube patterning lung alveolus development positive regulation of protein binding anterior/posterior axis specification negative regulation of transcription, DNA-templated positive regulation of epidermal cell differentiation branching morphogenesis of an epithelial tube trachea formation specification of animal organ position negative regulation of glomerular mesangial cell proliferation positive regulation of smooth muscle cell proliferation intermediate mesodermal cell differentiation pulmonary artery endothelial tube morphogenesis pituitary gland development positive regulation of cell death lung morphogenesis positive regulation of endothelial cell proliferation bud dilation involved in lung branching positive regulation of cardiac muscle fiber development negative regulation of striated muscle tissue development positive regulation of cell migration negative regulation of branch elongation involved in ureteric bud branching by BMP signaling pathway BMP signaling pathway involved in nephric duct formation positive regulation of pathway-restricted SMAD protein phosphorylation negative regulation of mesenchymal cell proliferation involved in ureter development mesoderm formation cellular response to growth factor stimulus glomerular capillary formation bronchus development positive regulation of endothelial cell migration multicellular organism development negative regulation of metanephric S-shaped body morphogenesis neural tube closure vasculature development embryonic morphogenesis protein localization to nucleus positive regulation of apoptotic process embryonic skeletal joint morphogenesis regulation of cell differentiation negative regulation of branching involved in ureteric bud morphogenesis mesodermal cell differentiation neuron fate commitment forebrain development cloacal septation bone development camera-type eye morphogenesis positive regulation of SMAD protein signal transduction negative regulation of glomerulus development embryonic hindlimb morphogenesis positive chemotaxis outflow tract morphogenesis odontogenesis negative regulation of transcription by RNA polymerase II epithelial to mesenchymal transition involved in endocardial cushion formation cardiac jelly development cellular response to BMP stimulus positive regulation of osteoblast differentiation epithelial tube branching involved in lung morphogenesis cardiac muscle cell differentiation apoptotic process involved in endocardial cushion morphogenesis muscular septum morphogenesis positive regulation of transcription by RNA polymerase II positive regulation of bone mineralization cardiac right ventricle morphogenesis outflow tract septum morphogenesis membranous septum morphogenesis aortic valve morphogenesis pulmonary valve morphogenesis endocardial cushion development endoderm development coronary vasculature development BMP signaling pathway involved in heart development pharyngeal arch artery morphogenesis positive regulation of cell proliferation involved in outflow tract morphogenesis negative regulation of extrinsic apoptotic signaling pathway regulation of pri-miRNA transcription by RNA polymerase II positive regulation of production of miRNAs involved in gene silencing by miRNA post-translational protein modification positive regulation of gene expression positive regulation of epithelial to mesenchymal transition positive regulation of cardiac neural crest cell migration involved in outflow tract morphogenesis regulation of signaling receptor activity positive regulation of cell population proliferation negative regulation of gene expression negative regulation of pri-miRNA transcription by RNA polymerase II regulation of apoptotic process regulation of MAPK cascade cell development growth Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 652 12159 Ensembl ENSG00000125378 ENSMUSG00000021835 UniProt P12644 P21275 RefSeq (mRNA) NM_001202 NM_130850 NM_130851 NM_007554 NM_001316360 RefSeq (protein) NP_001193 NP_570911 NP_001334841 NP_001334842 NP_001334843 NP_001334844 NP_001334845 NP_001334846 NP_570912 NP_001303289 NP_031580 Location (UCSC) n/a Chr 14: 46.62 – 46.63 Mb PubMed search [2] [3]
Wikidata
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Bone morphogenetic protein 4 is a protein that in humans is encoded by BMP4 gene.^[4][5] BMP4 is found on chromosome 14q22-q23.

BMP4 is a member of the bone morphogenetic protein family which is part of the transforming growth factor-beta superfamily. The superfamily includes large families of growth and differentiation factors. BMP4 is highly conserved evolutionarily. BMP4 is found in early embryonic development in the ventral marginal zone and in the eye, heart blood and otic vesicle.^[6]

Discovery

Bone morphogenetic proteins were originally identified by an ability of demineralized bone extract to induce endochondral osteogenesis in vivo in an extraskeletal site.

Function

BMP4 is a polypeptide belonging to the TGF-β superfamily of proteins. It, like other bone morphogenetic proteins, is involved in bone and cartilage development, specifically tooth and limb development and fracture repair. This particular family member plays an important role in the onset of endochondral bone formation in humans. It has been shown to be involved in muscle development, bone mineralization, and ureteric bud development.^[7]

BMP4 stimulates differentiation of overlying ectodermal tissue. ^[8]

Bone morphogenetic proteins are known to stimulate bone formation in adult animals. This is thought that inducing osteoblastic commitment and differentiation of stem cells such as mesenchymal stem cells.^{[citation needed]}BMPs are known to play a large role in embryonic development. In the embryo BMP4 helps establish dorsal-ventral axis formation in Xenopus frog through inducing ventral mesoderm. In mice targeted inactivation of BMP4 disrupts mesoderm from forming.^[9] As well establishes dorsal-ventral patterning of the developing neural tube with the help of BMP7, and inducing dorsal characters.^{[citation needed]}

BMP4 also limits the extent to which neural differentiation in Xenopus embryos occurs by inducing epidermis formation rather than neural tissue. They can aid in inducing the lateral characteristics in somites. Somites are required for the development of cartilage, bone, dermis on the dorsal side of the body, thoracic muscles and muscles within limbs.^[10] BMP4 helps in the patterning of the developing head though inducing apoptosis of the neural crest cells; this is done in the hindbrain. ^[11]

In adult, BMP4 is important for the neurogenesis (i.e., the generation of new neurons) that occurs throughout life in two neurogenic niches of the brain, the dentate gyrus of the hippocampus and the subventricular zone (SVZ) adjacent to lateral ventricles. In these niches new neurons are continuously generated from stem cells. In fact it has been shown that in the dentate gyrus BMP4 maintains neural stem cells in quiescence, thus preventing the depletion of the pool of stem cells.^[12] In the SVZ , BMP-mediated signaling via Smad4 is required to initiate neurogenesis from adult neural stem cells and suppress the alternative fate of oligodendrogliogenesis.^[13] Moreover, it has been shown that in the SVZ BMP4 has a prodifferentiative effect, since it rescues a defect of terminal differentiation in SVZ neurospheres where the gene Tis21/BTG2 - required for terminal differentiation - has been deleted.^[14] Tis21 is a positive regulator of BMP4 expression in the SVZ.^[14]

BMP4 is important for bone and cartilage metabolism. The BMP4 signaling has been found in formation of early mesoderm and germ cells. Limb bud regulation and development of the lungs, liver, teeth and facial mesenchyme cells are other important functions attributed to BMP4 signaling.^[15] Digit formation is influenced by BMP4, along with other BMP signals. The interdigital mesenchyme exhibits BMP4, which prevents apoptosis of the region.^[16] Tooth formation relies on BMP4 expression, which induces Msx 1 and 2. These transcription factors turn the forming tooth to become and incisor.

BMP4 also plays important roles in adipose tissue: it is essential for white adipogenesis, and promotes adipocyte differentiation.^[17] Additionally, it is also important for brown fat, where it induces UCP1, related to non-shivering thermogenesis.^[17]

BMP4 secretion helps cause differentiation of the ureteric bud into the ureter.^[18]

BMP4 antagonizes organizer tissue and is expressed in early development in ectoderm and mesoderm tissue. Upon gastrulation, the transcription of BMP4 is limited to the ventrolateral marginal zone due to inhibition from the dorsalizing side of the developing embryo. BMP4 aids in ventralizing mesoderm, which guides the dorsal-ventral axis formation. In Xenopus BMP4 has been found to aid in formation of blood and blood islands. ^[19]

BMP4, initially expressed in the epidermis, is found in the roof plate during formation of the neural tube. A gradient of BMP signaling is found in opposition to a Sonic hedgehog, Shh, gradient. This expression of BMP4 patterns the dorsal neurons.^[20]

BMP4, in conjunction with FGF2, promote differentiation of stem cells to mesodermal lineages. After differentiation, BMP4 and FGF2 treated cells generally produces higher amounts of osteogenic and chondrogenic differentiation than untreated stem cells.^[21] Also in conjunction with FGF2 it can produce progenitor thyroid cells from pluripotent stem cells in mice and humans.^[22]

BMP4 has been shown to induce the expression of the Msx gene family, which is believed to be part of cartilage formation from somitic mesoderm.^[23]

BMP4, a paracrine growth factor, has been found in rat ovaries. BMP4, in conjunction with BMP7, regulate early ovarian follicle development and primordial-to-primary follicle transition. In addition, inhibition of BMP4 with antibodies has been shown to decrease overall ovary size. These results indicate that BMP4 may aid in survival and prevention of apoptosis in oocytes.^[15]

In birds, BMP4 has been shown to influence the beak size of Darwin's finches. Low amounts of BMP4 are correlated with low beak depths and widths. Conversely, high BMP4 expression makes high beak depths and widths. The genetic regulation of BMP4 provides the foundation for natural selection in bird beaks.^[24]

Protein structure

Yielding an active carboxy-terminal peptide of 116 residues, human bmp4 is initially synthesized as a forty percent residue preproprotein which is cleaved post translationally. BMP4 has seven residues which are conserved and glycosylated.^[25] The monomers are held with disulphide bridges and 3 pairs of cysteine amino acids. This conformation is called a “cystine knot”. BMP4 can form homodimers or heterodimers with similar BMPS. One example of this is BMP7. This ability to form homodimers or heterodimers gives the ability to have greater osteoinductive activity than just bmp4 alone.^[26] Not much is known yet about how BMPS interact with the extracellular matrix. As well little is known about the pathways which then degrade BMP4.

Inhibition

Inhibition of the BMP4 signal (by chordin, noggin, or follistatin) causes the ectoderm to differentiate into the neural plate. If these cells also receive signals from FGF, they will differentiate into the spinal cord; in the absence of FGF the cells become brain tissue.

While overexpression of BMP4 expression can lead to ventralization, inhibition with a dominant negative may result in complete dorsalization of the embryo or the formation of two axises.^[27]

It is important to note that mice in which BMP4 was completely inactivated usually died during gastrulation. It is thought that inactivation of human BMP4 would likely have the same effect. However, mutations which don't entirely inactivate BMP4 in humans can also have subtle effects phenotypically, and have been implicated in tooth agenesis as well as osteoporosis.^[28]

Isoforms

Alternative splicing in the 5' untranslated region of this gene has been described and three variants are described, all encoding an identical protein.^[29]

Molecular mechanisms

BMP4, as a member of the transforming growth factor-β (TGF-β) family binds to 2 different types of serine-threonine kinase receptors known as BMPR1 and BMPR2.^[30] Signal transduction via these receptors occurs via Smad and map kinase pathways to effect transcription of its target genes. In order for signal transduction to occur, both receptors must be functional. BMP is able to bind to BMPR2 without BMPR1 however, the affinity significantly increases in the presence of both receptors. BMPR1 is transphosphorylated via BMPR2 which induces downstream signalling within the cell, affecting transcription.^[30]

Smad signaling pathway

TGF-β family receptors most commonly use the Smad signaling pathway to tranduce signals.^[30] Type 2 receptors are responsible for activating type 1 receptors where their function involves the phosphorylation of R-Smads (Smad-1, Smad-5, Smad-8). Upon phosphorylation, formation of an R-SMAD complex in conjunction with common-partner Smad (co-Smad) occurs where it migrates to the nucleus. This signaling pathway is regulated by the small molecule inhibitor known as dorsomorphin which prevents the downstream effects of R-smads.^[30]

Map kinase (MAPK) signaling pathways

Mitogen activated protein kinases (MAPK) undergo phosphorylation via a signaling cascade where MAPKKK phosphorylates and activates MAPKK and MAPKK phosphorylates and activates MAPK which then induces an intracellular response.^[31] Activation of MAPKKK is through the interaction of mainly GTPases or another group of protein kinases. TGF-β receptors induce the MAPK signaling pathways of ERK, JNK and p38.^[31] BMP4 is also known to activate the ERK, JNK and p38 MAPK signalling pathways whilst have been found to act independently of Smad signaling pathways, are mostly active in conjunction with Smad.^[32] The activation of the ERK and JNK pathways acts to phosphorylate Smad and therefore regulate its activation. In addition to this, MAPK pathways may be able to directly affect Smad-interacting transcription factors via a JNK or p38 substrate that induces convergence of the two signaling pathways. This convergence is noted to consist mainly of cooperative behavior however, there is evidence to suggest that they may at times counteract each other. Furthermore, the balance that exists between the direct activation of these signaling pathways has a significant effect on TGF-β induced cellular responses.^[32]

Clinical significance

Increase in expression of BMP4 has been associated with a variety of bone diseases, including the heritable disorder Fibrodysplasia Ossificans Progressiva.^[33]

There is strong evidence from sequencing studies of candidate genes involved in clefting that mutations in the bone morphogenetic protein 4 (BMP4) gene may be associated in the pathogenesis of cleft lip and palate.^[34]

Eye development

Eyes are essential for organisms, especially terrestrial vertebrates, to observe prey and obstacles; this is critical for their survival. The formation of the eyes starts as optic vesicles and lens derived from the neuroectoderm. Bone morphogenic proteins are known to stimulate eye lens formation. During early development of eyes, the formation of the optic vesicle is essential in Mice and BMP4 expressed strongly in the optic vesicle and weakly in the surrounding mesenchyme and surface ectoderm. This concentration gradient of BMP4 in optic vesicle is critical for lens induction. Researcher, Dr. Furuta and Dr. Hogan found out that if they did a laser mutation on mice embryos and causing a BMP4 homozygous null mutation, this embryo will not develop the lens. They also did an in situ hybridization of the BMP4 gene showing green color and Sox2 gene in red which they thought it was involved in the lens formation as well. After they did these two in situ hybridizations in the mice embryos, they found that both green and red colors are found in the optic vesicle of the mice embryos. This indicated that BMP4 and Sox2 are expressed in the right place at the right time of the optic vesicle and prove that they have some essential functions for the lens induction. Furthermore, they did a follow-up experiment that by injecting BMP4 into the BMP4 homozygous mutant embryos rescued the lens formation (12). This indicated that BMP4 is definitely required for lens formation. However, researchers also found that some of the mutated mice cannot be rescued. They later found that those mutants lacked of Msx 2 which is activated by BMP4. The mechanism they predicted was that BMP4 will active Msx 2 in the optic vesicle and concentration combination of BMP4 and Msx2 together active Sox2 and the Sox2 is essential for lens differentiation.^[35]

Injection of Noggin into lens fiber cells in mice significantly reduces the BMP4 proteins in the cells. This indicates that Noggin is sufficient to inhibit the production of BMP4. Moreover, another inhibitor protein, Alk6 was found that blocked the BMP4 from activating the Msx2 which stopped lens differentiation .^[36] However, there are still a lot of unknown about the mechanism of inhibition on BMP4 and downstream regulation of Sox2. In the future, researchers are aiming to find out a more complete pathway of whole eye development and hoping one day, they can find a way to cure some genetic caused eye diseases.

Hair loss

Hair loss or known as alopecia is caused from the changing of hair follicle morphology and hair follicle cycling in an abnormal fashion.^[37] The cycles of hair follicles are that of growth, or anagen, regression or catagen, and rest or telogen.^[38] In mammals reciprocal epithelial and mesenchymal interactions control the development of hair. Genes such as BMP4 and BMP2 are both active within the precursors of the hair shaft. Specifically BMP4 is found in the dermal papilla. BMP4 is part of the signaling network which controls the development of hair. It is needed for the induction of biochemical pathways and signaling for regulating the differentiation of the hair shaft in the anagen hair follicle. This is done through controlling the expression of the transcription factors which regulate hair differentiation. It is still unclear however where BMPs act within the genetic network. The signaling of bmp4 may potentially control expression of terminal differentiation molecules such as keratins. Other regulators have been shown to control hair follicle development as well. HOXC13 and FOXN1 are considered important regulators because loss-of-function experiments show impaired hair shaft differentiation that doesn’t interfere in the hair follicle formation.^[39]

When BMP4 is expressed ectopically, within transgenic mice the hair follicle outer root sheath (ORS) the proliferation of the cell matrix is inhibited. BMP4 also activates hair keratin gene expression noting that BMP4 is important in the differentiation of the hair shaft. Noggin, a known inhibitor of BMP4, is found within the matrix cells of the hair bulb. Other important factors to consider in the development of hair is the expression of Shh (sonic hedgehog), BMP7, BMP2, WNT, and β-catenin as these are required in early stage morphogenesis.^[40]

Other genes which can inhibit or interact with BMP4 are noggin, follistatin, gremlin, which is all expressed in the developing hair follicles.^[41] In mice in which noggin is lacking, there are fewer hair follicles than on a normal mouse and the development of the follicle is inhibited. In chick embryos it is shown that ectopically expressed noggin produces enlarged follicles, and BMP4 signaling shows repressed placode fate in nearby cells.^[26] Noggin has also been shown during in vivo experiments to induce hair growth in post natal skin.^[42]

BMP4 is an important component of the biological pathways that involved regulating hair shaft differentiation within the anagen hair follicle. The strongest levels of expressed BMP4 are found within the medulla, hair shaft cells, distal hair matrix, and potential precursors of the cuticle. The two main methods which BMP4 inhibit expression of hair is through restricting growth factor expression in the hair matrix and antagonism between growth and differentiation signaling.^[40]

Pathways that regulate hair follicle formation and hair growth are key in developing therapeutic methods for hair loss conditions. Such conditions include the development of new follicles, changing the shape of characteristics of existing follicles, and the altering of hair growth in existing hair follicles. Furthermore, BMP4 and the pathway through which it works may provide therapeutic targets for the prevention of hair loss.^[38]

References

Further reading

External links

• BMPedia - the Bone Morphogenetic Protein Wiki^{[permanent dead link]}
• BMP4 human gene location in the UCSC Genome Browser.
• BMP4 human gene details in the UCSC Genome Browser.