CLIC4

Protein-coding gene in the species Homo sapiens
CLIC4
Available structures
PDBOrtholog search: PDBe RCSB
List of PDB id codes

2AHE, 2D2Z, 3OQS

Identifiers
AliasesCLIC4, CLIC4L, H1, MTCLIC, huH1, p64H1, chloride intracellular channel 4
External IDsOMIM: 606536 MGI: 1352754 HomoloGene: 8490 GeneCards: CLIC4
Gene location (Human)
Chromosome 1 (human)
Chr.Chromosome 1 (human)[1]
Chromosome 1 (human)
Genomic location for CLIC4
Genomic location for CLIC4
Band1p36.11Start24,745,382 bp[1]
End24,844,321 bp[1]
Gene location (Mouse)
Chromosome 4 (mouse)
Chr.Chromosome 4 (mouse)[2]
Chromosome 4 (mouse)
Genomic location for CLIC4
Genomic location for CLIC4
Band4|4 D3Start134,941,280 bp[2]
End135,000,125 bp[2]
RNA expression pattern
Bgee
HumanMouse (ortholog)
Top expressed in
  • saphenous vein

  • popliteal artery

  • vena cava

  • smooth muscle tissue

  • ascending aorta

  • right coronary artery

  • urethra

  • trigeminal ganglion

  • Achilles tendon

  • lower lobe of lung
Top expressed in
  • right lung

  • right lung lobe

  • ascending aorta

  • sciatic nerve

  • semi-lunar valve

  • left lung

  • atrioventricular valve

  • aortic valve

  • belly cord

  • primitive streak
More reference expression data
BioGPS




More reference expression data
Gene ontology
Molecular function
  • voltage-gated ion channel activity
  • protein binding
  • glutathione transferase activity
  • chloride channel activity
Cellular component
  • cytoplasm
  • integral component of membrane
  • cytosol
  • centrosome
  • membrane
  • cell-cell junction
  • nuclear matrix
  • microtubule cytoskeleton
  • plasma membrane
  • apical part of cell
  • intracellular anatomical structure
  • chloride channel complex
  • microtubule organizing center
  • microvillus
  • cell surface
  • cell junction
  • midbody
  • mitochondrion
  • actin cytoskeleton
  • perinuclear region of cytoplasm
  • extracellular exosome
  • cytoskeleton
  • cytoplasmic vesicle membrane
  • cytoplasmic vesicle
  • nucleus
Biological process
  • cellular response to calcium ion
  • cell differentiation
  • multicellular organism growth
  • regulation of ion transmembrane transport
  • endothelial cell morphogenesis
  • ion transport
  • keratinocyte differentiation
  • vacuolar acidification
  • negative regulation of cell migration
  • fertilization
  • establishment or maintenance of apical/basal cell polarity
  • chloride transport
  • branching morphogenesis of an epithelial tube
  • retina vasculature morphogenesis in camera-type eye
  • regulation of cytoskeleton organization
  • angiogenesis
  • glutathione metabolic process
  • chloride transmembrane transport
  • transport
  • ion transmembrane transport
Sources:Amigo / QuickGO
Orthologs
SpeciesHumanMouse
Entrez

25932

29876

Ensembl

ENSG00000169504

ENSMUSG00000037242

UniProt

Q9Y696

Q9QYB1

RefSeq (mRNA)

NM_013943

NM_013885

RefSeq (protein)

NP_039234

NP_038913

Location (UCSC)Chr 1: 24.75 – 24.84 MbChr 4: 134.94 – 135 Mb
PubMed search[3][4]
Wikidata
View/Edit HumanView/Edit Mouse

Chloride intracellular channel 4, also known as CLIC4,p644H1,HuH1, is a eukaryotic gene.[5]

Chloride channels are a diverse group of proteins that regulate fundamental cellular processes including stabilization of cell membrane potential, transepithelial transport, maintenance of intracellular pH, and regulation of cell volume. Chloride intracellular channel 4 (CLIC4) protein, encoded by the clic4 gene, is a member of the p64 family; the gene is expressed in many tissues. These channels are implicated in angiogenesis, pulmonary hypertension, cancer, and cardioprotection from ischemia-reperfusion injury. They exhibit an intracellular vesicular pattern in PANC-1 cells (pancreatic cancer cells).[5]

Binding partners

CLIC4 binds to dynamin I, α-tubulin, β-actin, creatine kinase and two 14-3-3 isoforms.[6]

See also

References

  1. ^ a b c GRCh38: Ensembl release 89: ENSG00000169504 – Ensembl, May 2017
  2. ^ a b c GRCm38: Ensembl release 89: ENSMUSG00000037242 – Ensembl, May 2017
  3. ^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  4. ^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  5. ^ a b "Entrez Gene: CLIC4 chloride intracellular channel 4".
  6. ^ Suginta W, Karoulias N, Aitken A, Ashley RH (October 2001). "Chloride intracellular channel protein CLIC4 (p64H1) binds directly to brain dynamin I in a complex containing actin, tubulin and 14-3-3 isoforms". Biochem. J. 359 (Pt1): 55–64. doi:10.1042/0264-6021:3590055. PMC 1222121. PMID 11563969.

Further reading

  • Berryman M, Bretscher A (2000). "Identification of a Novel Member of the Chloride Intracellular Channel Gene Family (CLIC5) That Associates with the Actin Cytoskeleton of Placental Microvilli". Mol. Biol. Cell. 11 (5): 1509–21. doi:10.1091/mbc.11.5.1509. PMC 14863. PMID 10793131.
  • Ponnalagu D, Hamilton S, Sanghvi S, Antelo D, Schwieterman N, Hansra I, Xu X, Gao E, Edwards JC, Bansal SS, Wold LE, Terentyev, D, Janssen PM, Hund TJ, Khan M, Kohut AR, Koch WJ, Singh H (2022). "CLIC4 localizes to mitochondrial-associated membranes and mediates cardioprotection". Science Advances. 8 (42): eabo1244. Bibcode:2022SciA....8O1244P. doi:10.1126/sciadv.abo1244. PMC 9586484. PMID 36269835.
  • Berryman MA, Goldenring JR (2004). "CLIC4 is enriched at cell-cell junctions and colocalizes with AKAP350 at the centrosome and midbody of cultured mammalian cells". Cell Motil. Cytoskeleton. 56 (3): 159–72. doi:10.1002/cm.10141. PMID 14569596.
  • Bohman S, Matsumoto T, Suh K, et al. (2006). "Proteomic analysis of vascular endothelial growth factor-induced endothelial cell differentiation reveals a role for chloride intracellular channel 4 (CLIC4) in tubular morphogenesis". J. Biol. Chem. 280 (51): 42397–404. doi:10.1074/jbc.M506724200. PMID 16239224.
  • Chuang JZ, Milner TA, Zhu M, Sung CH (1999). "A 29 kDa intracellular chloride channel p64H1 is associated with large dense-core vesicles in rat hippocampal neurons". J. Neurosci. 19 (8): 2919–28. doi:10.1523/JNEUROSCI.19-08-02919.1999. PMC 6782274. PMID 10191309.
  • Duncan RR, Westwood PK, Boyd A, Ashley RH (1997). "Rat brain p64H1, expression of a new member of the p64 chloride channel protein family in endoplasmic reticulum". J. Biol. Chem. 272 (38): 23880–6. doi:10.1074/jbc.272.38.23880. PMID 9295337.
  • Edwards JC (1999). "A novel p64-related Cl− channel: subcellular distribution and nephron segment-specific expression". Am. J. Physiol. 276 (3 Pt 2): F398–408. doi:10.1152/ajprenal.1999.276.3.F398. PMID 10070163. S2CID 19105320.
  • Qian Z, Okuhara D, Abe MK, Rosner MR (1999). "Molecular cloning and characterization of a mitogen-activated protein kinase-associated intracellular chloride channel". J. Biol. Chem. 274 (3): 1621–7. doi:10.1074/jbc.274.3.1621. PMID 9880541.
  • Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The Status, Quality, and Expansion of the NIH Full-Length cDNA Project: The Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
  • Hartley JL, Temple GF, Brasch MA (2001). "DNA Cloning Using In Vitro Site-Specific Recombination". Genome Res. 10 (11): 1788–95. doi:10.1101/gr.143000. PMC 310948. PMID 11076863.
  • Ota T, Suzuki Y, Nishikawa T, et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs". Nat. Genet. 36 (1): 40–5. doi:10.1038/ng1285. PMID 14702039.
  • Rønnov-Jessen L, Villadsen R, Edwards JC, Petersen OW (2002). "Differential Expression of a Chloride Intracellular Channel Gene, CLIC4, in Transforming Growth Factor-β1-Mediated Conversion of Fibroblasts to Myofibroblasts". Am. J. Pathol. 161 (2): 471–80. doi:10.1016/S0002-9440(10)64203-4. PMC 1850723. PMID 12163372.
  • Rual JF, Venkatesan K, Hao T, et al. (2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature. 437 (7062): 1173–8. Bibcode:2005Natur.437.1173R. doi:10.1038/nature04209. PMID 16189514. S2CID 4427026.
  • Shiio Y, Suh KS, Lee H, et al. (2006). "Quantitative proteomic analysis of myc-induced apoptosis: a direct role for Myc induction of the mitochondrial chloride ion channel, mtCLIC/CLIC4". J. Biol. Chem. 281 (5): 2750–6. doi:10.1074/jbc.M509349200. PMID 16316993.
  • Simpson JC, Wellenreuther R, Poustka A, et al. (2001). "Systematic subcellular localization of novel proteins identified by large-scale cDNA sequencing". EMBO Rep. 1 (3): 287–92. doi:10.1093/embo-reports/kvd058. PMC 1083732. PMID 11256614.
  • Singh H, Ashley RH (2007). "CLIC4 (p64H1) and its putative transmembrane domain form poorly selective, redox-regulated ion channels". Mol. Membr. Biol. 24 (1): 41–52. doi:10.1080/09687860600927907. PMID 17453412. S2CID 9986497.
  • Singh H, Cousin MA, Ashley RH (2007). "Functional reconstitution of mammalian 'chloride intracellular channels' CLIC1, CLIC4 and CLIC5 reveals differential regulation by cytoskeletal actin". FEBS J. 274 (24): 6306–6316. doi:10.1111/j.1742-4658.2007.06145.x. PMID 18028448. S2CID 22494250.
  • Singh H (2010). "Two decades with dimorphic Chloride Intracellular Channels (CLICs)". FEBS Letters. 584 (10): 2112–21. doi:10.1016/j.febslet.2010.03.013. PMID 20226783.
  • Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. Bibcode:2002PNAS...9916899M. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
  • Suginta W, Karoulias N, Aitken A, Ashley RH (2003). "Chloride intracellular channel protein CLIC4 (p64H1) binds directly to brain dynamin I in a complex containing actin, tubulin and 14-3-3 isoforms". Biochem. J. 359 (Pt 1): 55–64. doi:10.1042/0264-6021:3590055. PMC 1222121. PMID 11563969.
  • Valenzuela SM, Martin DK, Por SB, et al. (1997). "Molecular cloning and expression of a chloride ion channel of cell nuclei". J. Biol. Chem. 272 (19): 12575–82. doi:10.1074/jbc.272.19.12575. PMID 9139710.
  • Wick M, Bürger C, Brüsselbach S, et al. (1994). "Identification of serum-inducible genes: different patterns of gene regulation during G0-->S and G1-->S progression". J. Cell Sci. 107 (1): 227–39. doi:10.1242/jcs.107.1.227. PMID 8175911.
  • Wiemann S, Arlt D, Huber W, et al. (2004). "From ORFeome to Biology: A Functional Genomics Pipeline". Genome Res. 14 (10B): 2136–44. doi:10.1101/gr.2576704. PMC 528930. PMID 15489336.
  • Wiemann S, Weil B, Wellenreuther R, et al. (2001). "Toward a Catalog of Human Genes and Proteins: Sequencing and Analysis of 500 Novel Complete Protein Coding Human cDNAs". Genome Res. 11 (3): 422–35. doi:10.1101/gr.GR1547R. PMC 311072. PMID 11230166.

External links

This article incorporates text from the United States National Library of Medicine, which is in the public domain.


  • v
  • t
  • e
  • 2ahe: Crystal structure of a soluble form of CLIC4. intercellular chloride ion channel
    2ahe: Crystal structure of a soluble form of CLIC4. intercellular chloride ion channel
  • 2d2z: Crystal structure of Soluble Form Of CLIC4
    2d2z: Crystal structure of Soluble Form Of CLIC4
  • v
  • t
  • e
Ligand-gated
Voltage-gated
Constitutively active
Proton-gated
Voltage-gated
Calcium-activated
Inward-rectifier
Tandem pore domain
Voltage-gated
Miscellaneous
Cl−: Chloride channel
H+: Proton channel
M+: CNG cation channel
M+: TRP cation channel
H2O (+ solutes): Porin
Cytoplasm: Gap junction
By gating mechanism
Ion channel class
see also disorders


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